Please cite as: CSH Protocols; 2007; doi:10.1101/pdb.prot4842

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Genetic Variation: A Laboratory Manual
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Genotyping by Dideoxy Resequencing

Edwin Cuppen

Hubrecht Laboratorium, 3584 CT Utrecht, The Netherlands

Corresponding author (ecuppen{at}niob.knaw.nl)


INTRODUCTION

This protocol describes dideoxy resequencing of PCR fragments, which is a quick and flexible genotyping technique. This strategy is used to identify and confirm the presence of heterozygous single-nucleotide polymorphisms (SNPs) within a genomic region of interest.


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