Please cite as: CSH Protocols; 2007; doi:10.1101/pdb.top19

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Genetic Variation: A Laboratory Manual
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topic_introductionTopic Introduction

Preparing DNA from Mammalian Sources for Genotyping

Amrik Sahota1,3, Andrew I. Brooks1,2, and Jay A. Tischfield1

1Rutgers University Cell and DNA Repository, Department of Genetics, Piscataway, NJ 08854-8082, USA
2Bionomics Research and Technology Center, Environmental and Occupational Health Sciences Institute, University of Medicine and Dentistry of New Jersey--Robert Wood Johnson Medical School, Piscataway, NJ 08854-5635, USA

3Corresponding author (sahota{at}biology.rutgers.edu)


INTRODUCTION

The availability of high-quality DNA from a large number of individuals is a prerequisite for the success of genetic variation studies. This requirement has spurred major technological advances in DNA extraction methodologies. Twenty years ago, large-scale manual extractions took >3 d to complete. Large-scale preparations can now be completed within 3 h using automated extraction platforms, without the need for toxic reagents and often with higher yields than in the past. This article introduces methods and considerations for the extraction of DNA for genotyping, and for the determination of DNA quantity and quality.


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