Please cite as: CSH Protocols; 2007; doi:10.1101/pdb.prot4829

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Genetic Variation: A Laboratory Manual

protocolProtocol

Preparing DNA from Cell Pellets for Genotyping

Amrik Sahota1,3, Andrew I. Brooks1,2, and Jay A. Tischfield1

1Rutgers University Cell and DNA Repository, Department of Genetics, Piscataway, NJ 08854-8082, USA
2Bionomics Research and Technology Center, Environmental and Occupational Health Sciences Institute, University of Medicine and Dentistry of New Jersey--Robert Wood Johnson Medical School, Piscataway, NJ 08854-5635, USA

3Corresponding author (sahota{at}biology.rutgers.edu)


INTRODUCTION

This protocol describes large-scale DNA extraction from cell pellets using either in-house or commercial (PUREGENE) reagents. The cell pellets are prepared from human lymphoblast cell lines (LCLs) that have been established using the Epstein-Barr virus (EBV).


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Preparing DNA from Mammalian Sources for Genotyping
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