Please cite as: CSH Protocols; 2007; doi:10.1101/pdb.prot4770

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A Behavioral Stress Assay in Drosophila Larvae

Elizabeth A. Daubert and Barry G. Condron1

Department of Biology, University of Virginia, Charlottesville, Virginia 22903-4328, USA

1Corresponding author (Condron{at}virginia.edu)


INTRODUCTION

This protocol describes a simple behavioral assay designed to test the response of Drosophila larvae to a homeostatic insult: bright light. Treating normally photophobic larvae with bright light before placing them near a target food source reveals a greater latency to reach the target when compared to controls not receiving light treatment. This effect is reversible given a recovery period after light treatment, and it may reveal a method by which to measure behavioral plasticity and stress responses in fruit fly larvae.


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