Please cite as: CSH Protocols; 2007; doi:10.1101/pdb.prot4753

This Protocol
Right arrow Full Text
Right arrow Update/discuss this protocolDiscussion icon
Right arrow Alert me when this protocol is cited
Right arrow Alert me when comments are published
Right arrow Alert me if a correction is posted
Services
Right arrow Similar protocols in this database
Right arrow Alert me to new releases of protocols
Right arrow Save to Personal Folders
Right arrow Download to citation manager
Right arrow Printer-friendly versionPrinter-friendly version
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sive, H. L.
Right arrow Articles by Harland, R. M.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Sive, H. L.
Right arrow Articles by Harland, R. M.
Related Collections
Right arrow Xenopus
Right arrow CSHL Xenopus Course
Right arrow Developmental Biology
Right arrow Cell Biology, general
Right arrow Laboratory Organisms, general
Right arrowRelated Protocols
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Legend icon

protocolProtocol

Cortical Isolation from Xenopus laevis Oocytes and Eggs

Hazel L. Sive, Robert M. Grainger, and Richard M. Harland

This protocol was adapted from "Microdissection," Chapter 10, in Early Development of Xenopus laevis, by Hazel L. Sive, Robert M. Grainger, and Richard M. Harland. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2000.


INTRODUCTION

In Xenopus laevis, the cortex is the layer of gelatinous cytoplasm that lies just below the plasma membrane of the egg. Rotation of the cortex relative to the deeper cytoplasm soon after fertilization is intimately linked to normal dorsal axis specification. The cortex can be dissected from the egg to analyze its composition and activity or to clone associated RNAs. This protocol describes a procedure for isolating the vegetal cortex of the fertilized egg.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?

Related Protocols

Dejellying Xenopus laevis Embryos
Hazel L. Sive, Robert M. Grainger, and Richard M. Harland
CSH Protocols 2007: 4731. [Abstract] [Full Text]

Removing the Vitelline Membrane from Xenopus laevis Embryos
Hazel L. Sive, Robert M. Grainger, and Richard M. Harland
CSH Protocols 2007: 4732. [Abstract] [Full Text]

Embryo Dissection and Micromanipulation Tools
Hazel L. Sive, Robert M. Grainger, and Richard M. Harland
CSH Protocols 2007: 7. [Abstract] [Full Text]

Microdissection: Explant and Transplant Assays in Xenopus laevis
Hazel L. Sive, Robert M. Grainger, and Richard M. Harland
CSH Protocols 2007: 10. [Abstract] [Full Text]