Please cite as: CSH Protocols; 2007; doi:10.1101/pdb.prot4346

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Inoculation and Passaging of Mammalian Cell Suspension Cultures

Gretchen J. Darlington

This protocol was adapted from "Growth and Manipulation of Cells in Culture," Chapter 2, in Cells (eds. Spector et al.). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 1998.


INTRODUCTION

This protocol describes the growth and passaging of mammalian cells in suspension culture using a simple dilution procedure. In situations in which total replacement of medium is desired, the cells can be gently pelleted by centrifugation and resuspended in the appropriate medium.


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