Please cite as: CSH Protocols; 2007; doi:10.1101/pdb.prot4735

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Isolating Xenopus laevis Testes

Hazel L. Sive, Robert M. Grainger, and Richard M. Harland

This protocol was adapted from "Obtaining Embryos," Chapter 5, in Early Development of Xenopus laevis by Hazel L. Sive, Robert M. Grainger, and Richard M. Harland. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2000.


INTRODUCTION

This protocol describes a method of isolating Xenopus laevis testes for use in in vitro fertilization. The testes from one male Xenopus contain sufficient sperm to fertilize several thousand eggs.


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