Please cite as: CSH Protocols; 2007; doi:10.1101/pdb.prot4657

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Microinjection of Protein Samples

Yulia Komarova, John Peloquin, and Gary Borisy

This protocol was adapted from "Microinjection of Fluorophore-Labeled Proteins," Chapter 5, in Live Cell Imaging (eds. Goldman and Spector). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2005.


INTRODUCTION

This protocol describes a method for microinjecting proteins into the nucleus or cytoplasm of adherent cells. Microinjection equipment can be obtained from a number of suppliers; this protocol has been used with the Narishige IM-200 air pressure regulator and the Leitz micromanipulator. Using this system, it is possible to microinject a constant volume within a 50% difference among cells.


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