Please cite as: CSH Protocols; 2007; doi:10.1101/pdb.prot4643

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Denaturing Protein Immunoprecipitation from Yeast

William P. Tansey

Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA

Corresponding author (tansey{at}cshl.edu)


INTRODUCTION

Where possible, it is preferable to use denaturing IPs to recover labeled proteins from pulse-chase experiments. The use of denaturing IPs reduces background and simplifies protein harvest. Yeast are boiled in an SDS-containing solution to liberate total proteins. This is also much safer than bead-beating radioactive yeast samples.


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