Please cite as: CSH Protocols; 2007; doi:10.1101/pdb.prot4619

This Protocol
Right arrow Full Text
Right arrow Update/discuss this protocolDiscussion icon
Right arrow Alert me when this protocol is cited
Right arrow Alert me when comments are published
Right arrow Alert me if a correction is posted
Services
Right arrow Similar protocols in this database
Right arrow Alert me to new releases of protocols
Right arrow Save to Personal Folders
Right arrow Download to citation manager
Right arrow Printer-friendly versionPrinter-friendly version
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tansey, W. P.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Tansey, W. P.
Related Collections
Right arrow Molecular Biology, general
Right arrow Proteins and Proteomics, general
Right arrow Antibodies, general
Right arrow Immunoprecipitation
Right arrow Protein: Protein Interactions, general
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Legend icon

protocolProtocol

Denaturing Protein Immunoprecipitation from Mammalian Cells

William P. Tansey

Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA

Corresponding author (tansey{at}cshl.edu)


INTRODUCTION

This protocol describes a denaturing immunoprecipitation (IP) for mammalian cells. Where possible, we prefer to use denaturing IPs to recover labeled proteins from pulse-chase experiments. The use of denaturing IPs reduces background considerably.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?