Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4666

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Transformation of Agrobacterium Using the Freeze-Thaw Method

Detlef Weigel and Jane Glazebrook

This protocol was adapted from "How to Transform Arabidopsis," Chapter 5, in Arabidopsis by Detlef Weigel and Jane Glazebrook. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2002.


INTRODUCTION

This protocol describes a method for transforming Agrobacterium with plasmid DNA using a freeze-thaw technique. Although the transformation efficiency for Agrobacterium is lower than that for Escherichia coli, it is possible to obtain adequate numbers of transformants with this technique.


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