Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4613

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6-Azauracil Sensitivity Assay for Yeast

William P. Tansey

Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA

Corresponding author (tansey{at}cshl.edu)


INTRODUCTION

Treatment of yeast with 6-Azauracil (6AU) leads to a reduction of intracellular GTP levels. The reduction in GTP levels is not itself lethal, but can block yeast growth when combined with mutations that affect transcriptional elongation. 6AU sensitivity thus can be used as a crude assay to test for mutations that affect transcriptional elongation. The assay described here requires growing saturated cultures of yeast, counting, and spotting serial dilutions of yeast on both CSM and CSM + 6AU plates.


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