Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4645

This Protocol
Right arrow Full Text
Right arrow Update/discuss this protocolDiscussion icon
Right arrow Alert me when this protocol is cited
Right arrow Alert me when comments are published
Right arrow Alert me if a correction is posted
Services
Right arrow Similar protocols in this database
Right arrow Alert me to new releases of protocols
Right arrow Save to Personal Folders
Right arrow Download to citation manager
Right arrow Printer-friendly versionPrinter-friendly version
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Verveer, P. J.
Right arrow Articles by Bastiaens, P. I.H.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Verveer, P. J.
Right arrow Articles by Bastiaens, P. I.H.
Related Collections
Right arrow Proteins and Proteomics, general
Right arrow Protein Imaging, general
Right arrow Imaging of Protein: Protein Interactions
Right arrow Protein: Protein Interactions, general
Right arrow Imaging/Microscopy, general
Right arrowRelated Protocol
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Legend icon

protocolProtocol

Imaging Protein Interactions by FRET Microscopy: Labeling Proteins with Fluorescent Dyes

Peter J. Verveer, Oliver Rocks, Ailsa G. Harpur, and Philippe I.H. Bastiaens

This protocol was adapted from "Imaging Protein Interactions by FRET Microscopy," Chapter 32, in Protein-Protein Interactions (eds. Golemis and Adams). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2005.


INTRODUCTION

This protocol provides a method for labeling proteins, such as antibodies and purified recombinant proteins, with succinimide esters of sulfoindocyanine (Cy) dyes. Cy dyes covalently bind to free amino groups (the {alpha}-amino-terminal or {epsilon}-amino groups on lysine side chains).


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?

Related Protocol

Imaging Protein Interactions by FRET Microscopy: Cell Preparation for FRET Analysis
Peter J. Verveer, Oliver Rocks, Ailsa G. Harpur, and Philippe I.H. Bastiaens
CSH Protocols 2006: 4646. [Abstract] [Full Text]