Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4616

This Protocol
Right arrow Full Text
Right arrow Update/discuss this protocolDiscussion icon
Right arrow Alert me when this protocol is cited
Right arrow Alert me when comments are published
Right arrow Alert me if a correction is posted
Services
Right arrow Similar protocols in this database
Right arrow Alert me to new releases of protocols
Right arrow Save to Personal Folders
Right arrow Download to citation manager
Right arrow Printer-friendly versionPrinter-friendly version
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tansey, W. P.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Tansey, W. P.
Related Collections
Right arrow Molecular Biology, general
Right arrow Proteins and Proteomics, general
Right arrow Protein Classification and Structure Prediction
Right arrowRelated Protocols
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Legend icon

protocolProtocol

Detection of Ubiquitylated Proteins in Mammalian Cells

William P. Tansey

Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA

Corresponding author (tansey{at}cshl.edu)


INTRODUCTION

This assay is performed to detect ubiquitylated proteins in transfected mammalian cells. Twenty-four to 40 hours after transfection, cells are harvested, total ubiquitylated proteins are recovered by nickel-affinity chromatography, and specific proteins are detected by Western blotting.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?

Related Protocols

Calcium-phosphate-mediated Transfection of Eukaryotic Cells with Plasmid DNAs
Joseph Sambrook and David W. Russell
CSH Protocols 2006: 3871. [Extract] [Full Text]

SDS-Polyacrylamide Gel Electrophoresis of Proteins
Joseph Sambrook and David W. Russell
CSH Protocols 2006: 4540. [Abstract] [Full Text]