Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.ip23

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Membrane Trafficking and Organelle Reagents

Jennifer Lippincott-Schwartz and Erik Lee Snapp

This information panel was adapted from "Imaging of Organelle Membrane Systems and Membrane Traffic in Living Cells," Chapter 32, in Live Cell Imaging: A Laboratory Manual (eds. Goldman and Spector). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2005.

The first 100 words of the full text of this article appear below.


RELATED INFORMATION

This information panel is associated with the protocol Imaging of Organelle Membrane Systems and Membrane Traffic in Living Cells.


Drugs

In this section, several common drugs, their targets, and protocols are described for studying organelle distribution and trafficking. The drugs are readily available from general suppliers, including Sigma, Roche, and Calbiochem. All drugs should be aliquoted and stored for single use or made freshly, when noted.

Brefeldin A

Brefeldin A (BFA) is a fungal metabolite that disrupts the function of the small GTPase Arf1, whose active state is necessary for maintaining the Golgi apparatus. Adding BFA to cells causes the Golgi to . . . [Full Text of this Article]

Microtubule Disruptors: Nocodazole and Colchicine

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Aluminum Fluoride

ATP Depletion

Protein Synthesis Inhibitors

N-Ethylmaleimide

Temperature Blocks

Dyes


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Related Protocol

Imaging of Organelle Membrane Systems and Membrane Traffic in Living Cells
Jennifer Lippincott-Schwartz and Erik Lee Snapp
CSH Protocols 2006: 4603. [Abstract] [Full Text]