Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.ip21

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Selection of Appropriate Imaging Equipment and Methodology for Live Cell Imaging in Drosophila

Ilan Davis and Richard M. Parton

This information panel was adapted from "Time-Lapse Cinematography in Living Drosophila Tissues," Chapter 21, in Live Cell Imaging: A Laboratory Manual (eds. Goldman and Spector). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2005.


INTRODUCTION

The wide range of microscopy equipment and techniques are truly bewildering and ever-expanding. In the past two decades, there have been many revolutions in light microscopy techniques made possible by improvements in optics, detector technology, and computers. Furthermore, there is no indication that the rate of development of new equipment is slowing down. Here we attempt to provide an overview of available options and important considerations applicable to imaging Drosophila cells and tissues.


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