Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.ip18

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Live Imaging of Caenorhabditis elegans: Observation of Nematodes and Data Collection

Benjamin Podbilewicz and Yosef Gruenbaum

Adapted from "Live Imaging of Caenorhabditis elegans," Chapter 20, in Live Cell Imaging: A Laboratory Manual (eds. Goldman and Spector). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2005.

The first 100 words of the full text of this article appear below.


OBSERVATION OF NEMATODES

Live nematodes can be studied at all levels of microscopic resolution (for examples and information pertaining to the preparation of Caenorhabditis elegans specimens, see Live Imaging of Caenorhabditis elegans: Examples and Live Imaging of Caenorhabditis elegans: Preparation of Samples). At low magnification, dissection microscopes are useful, whereas at higher magnification and resolution, a wide range of optical methods can be used, including differential interference contrast (DIC) optics, phase contrast, fluorescence, polarized light, confocal, and dark field. However, DIC optics are used for most applications. Some other helpful recommendations for observation include the following:


DATA COLLECTION

Photographic Records

Video Microscopy

Multiple-Focal-Plane Time-Lapse Recording Systems

Digital Imaging


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Live Imaging of Caenorhabditis elegans: Examples
Benjamin Podbilewicz and Yosef Gruenbaum
CSH Protocols 2006: 19. [Extract] [Full Text]

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Live Imaging of Caenorhabditis elegans: Preparation of Samples
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