Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.ip17

This Protocol
Right arrow Full Text
Right arrow Update/discuss this protocolDiscussion icon
Right arrow Alert me when this protocol is cited
Right arrow Alert me when comments are published
Right arrow Alert me if a correction is posted
Services
Right arrow Similar protocols in this database
Right arrow Alert me to new releases of protocols
Right arrow Save to Personal Folders
Right arrow Download to citation manager
Right arrow Printer-friendly versionPrinter-friendly version
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Verveer, P. J.
Right arrow Articles by Bastiaens, P. I.H.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Verveer, P. J.
Right arrow Articles by Bastiaens, P. I.H.
Related Collections
Right arrow Proteins and Proteomics, general
Right arrow Protein Imaging, general
Right arrow Imaging of Protein: Protein Interactions
Right arrow Protein: Protein Interactions, general
Right arrow Imaging/Microscopy, general
Right arrowRelated Article
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Legend icon

information_panelInformation Panel

FLIM Measurements and Frequency Domain FLIM Data Analysis

Peter J. Verveer, Oliver Rocks, Ailsa G. Harpur, and Philippe I.H. Bastiaens

This information panel was adapted from "Imaging Protein Interactions by FRET Microscopy," Chapter 32, in Protein-Protein Interactions (eds. Golemis and Adams). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2005.

The first 100 words of the full text of this article appear below.


RELATED INFORMATION

For more information about the photophysical principles of FRET and the derivations of the equations below, see Measuring FRET by Sensitized Emission.

FLIM Measurements

FRET reduces the fluorescence lifetime of the donor fluorophore ({tau}, a measure of the excited-state duration), since it depopulates its excited state, an effect that is also manifest as a reduction in the donor quantum yield (the ratio of the number of fluorescence photons emitted compared to the number of photons absorbed). FRET can be detected purely via donor fluorescence because {tau} is an intrinsic fluorescence parameter; that is, it is independent of probe concentration and light . . . [Full Text of this Article]

Frequency Domain FLIM Data Analysis

Figure Removed (Available Only in the Full Text)
View larger version (48K):



  		-->
-->


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?

Related Article

Measuring FRET by Sensitized Emission
Peter J. Verveer, Oliver Rocks, Ailsa G. Harpur, and Philippe I.H. Bastiaens
CSH Protocols 2006: 14. [Extract] [Full Text]