Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4556

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Digestion of Insoluble Protein Fractions for Multidimensional Protein Identification Technology (MuDPIT) Analysis

David M. Schieltz and Michael P. Washburn

This protocol was adapted from "The Use of Mass Spectrometry in Proteomics," Chapter 8 in Proteins and Proteomics (ed. Simpson). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.


INTRODUCTION

Proteins associated with insoluble particulate matter from a whole-cell lysate, or carefully prepared membrane samples, can be analyzed using MuDPIT analysis. However, the preliminary steps provided in this protocol are required to prepare a usable complex protein mixture from these types of samples.


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Related Protocol

Analysis of Complex Protein Mixtures Using Multidimensional Protein Identification Technology (MuDPIT)
David M. Schieltz and Michael P. Washburn
CSH Protocols 2006: 4555. [Abstract] [Full Text]