Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4552

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Modification of Lysyl Side Chains Using Citraconic Anhydride

Richard J. Simpson

This protocol was adapted from "Peptide Mapping and Sequence Analysis of Gel-Resolved Proteins," Chapter 7 in Proteins and Proteomics (ed. Simpson). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 2003.


INTRODUCTION

Reversible masking of {epsilon}-amino groups of lysine is a valuable procedure for limiting trypsin hydrolysis of proteins to arginine peptide bonds. Citraconic anhydride is a reversible blocking agent of lysyl side chains. A major advantage of citraconylation is the ease with which the blocking group can be removed under conditions that will not lead to protein denaturation.


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