Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4500

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Isolation and Purification of Tetrahymena Nuclei

Melody T. Sweet and C. David Allis

This protocol was adapted from "Culture and Manipulation of Tetrahymena," Chapter 18, in Cells (eds. Spector et al.). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 1998. This three-volume set is now out of print; however, some of the microscopy methods were republished in Basic Methods in Microscopy, by David L. Spector and Robert D. Goldman.


INTRODUCTION

The procedures described below allow the isolation of nuclei from all stages of the Tetrahymena life cycle in high yield with a high degree of purity. This method gives highly purified populations of both micronuclei and macronuclei.


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