Please cite as: CSH Protocols; 2006; doi:10.1101/pdb.prot4497

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Freezing and Thawing Tetrahymena Cultures

Melody T. Sweet and C. David Allis

This protocol was adapted from "Culture and Manipulation of Tetrahymena," Chapter 18, in Cells (eds. Spector et al.). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA,1998. This 3-volume set is now out of print; however, some of the microscopy methods were republished in Basic Methods in Microscopy, by David L. Spector and Robert D. Goldman.


INTRODUCTION

The method for freezing Tetrahymena has undergone major modifications over the years. The protocol given below was optimized by P.J. Bruns.


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