Protein Expression and Interactions

Culture of HEK293-EBNA1 Cells for Production of Recombinant Proteins
Roseanne Tom, Louis Bisson, and Yves Durocher
CSH Protocols; 2008; doi:10.1101/pdb.prot4976 [Abstract] [Full text]

Transfection of HEK293-EBNA1 Cells in Suspension with Linear PEI for Production of Recombinant Proteins
Roseanne Tom, Louis Bisson, and Yves Durocher
CSH Protocols; 2008; doi:10.1101/pdb.prot4977 [Abstract] [Full text]

Transfection of Adherent HEK293-EBNA1 Cells in a Six-Well Plate with Branched PEI for Production of Recombinant Proteins
Roseanne Tom, Louis Bisson, and Yves Durocher
CSH Protocols; 2008; doi:10.1101/pdb.prot4978 [Abstract] [Full text]

Transfection of HEK293-EBNA1 Cells in Suspension with 293fectin for Production of Recombinant Proteins
Roseanne Tom, Louis Bisson, and Yves Durocher
CSH Protocols; 2008; doi:10.1101/pdb.prot4979 [Abstract] [Full text]

Stable Isotope Labeling of Mammals (SILAM)
Daniel B. McClatchy and John R. Yates, III
CSH Protocols; 2008; doi:10.1101/pdb.prot4940 [Abstract] [Full text]

Identification of Protein-Protein Interactions with Glutathione-S-Transferase (GST) Fusion Proteins
Margret B. Einarson, Elena N. Pugacheva, and Jason R. Orlinick
CSH Protocols; 2007; doi:10.1101/pdb.top11 [Abstract] [Full text]

GST Pull-down
Margret B. Einarson, Elena N. Pugacheva, and Jason R. Orlinick
CSH Protocols; 2007; doi:10.1101/pdb.prot4757 [Abstract] [Full text]

Far Western: Labeling GST Fusion Proteins
Margret B. Einarson, Elena N. Pugacheva, and Jason R. Orlinick
CSH Protocols; 2007; doi:10.1101/pdb.prot4758 [Abstract] [Full text]

Far Western: Probing Membranes
Margret B. Einarson, Elena N. Pugacheva, and Jason R. Orlinick
CSH Protocols; 2007; doi:10.1101/pdb.prot4759 [Abstract] [Full text]

Affinity Purification of Interacting Proteins from Cell Lysates
Manuel Baca and Jian-Guo Zhang
CSH Protocols; 2007; doi:10.1101/pdb.prot4593 [Abstract] [Full text]