Preparation of Cellular and Subcellular Extracts

In Vivo Isotopic Labeling of Proteins for Quantitative Proteomics
Yoshiya Oda
CSH Protocols; 2007; doi:10.1101/pdb.prot4743 [Abstract] [Full text]

Preparation of Membrane Proteins from Drosophila Embryos
Claire X. Zhang and Tao-shih Hsieh
CSH Protocols; 2007; doi:10.1101/pdb.prot4711 [Abstract] [Full text]

Affinity Purification of Interacting Proteins from Cell Lysates
Manuel Baca and Jian-Guo Zhang
CSH Protocols; 2007; doi:10.1101/pdb.prot4593 [Abstract] [Full text]

Freeze-Thaw Lysis for Extraction of Proteins from Mammalian Cells
William P. Tansey
CSH Protocols; 2006; doi:10.1101/pdb.prot4614 [Abstract] [Full text]

Isolation and Purification of Tetrahymena Nuclei
Melody T. Sweet and C. David Allis
CSH Protocols; 2006; doi:10.1101/pdb.prot4500 [Abstract] [Full text]

Lysing Tissue-Culture Cells for Immunoprecipitation
Ed Harlow and David Lane
CSH Protocols; 2006; doi:10.1101/pdb.prot4531 [Abstract] [Full text]

Immunoprecipitation: Lysing Bacteria by Sonication
Ed Harlow and David Lane
CSH Protocols; 2006; doi:10.1101/pdb.prot4532 [Abstract] [Full text]

Immunoprecipitation: Lysing Yeast Cells Using Glass Beads
Ed Harlow and David Lane
CSH Protocols; 2006; doi:10.1101/pdb.prot4533 [Abstract] [Full text]

Immunoprecipitation: Denaturing Lysis
Ed Harlow and David Lane
CSH Protocols; 2006; doi:10.1101/pdb.prot4534 [Abstract] [Full text]

Immunoprecipitation: Preclearing the Lysate
Ed Harlow and David Lane
CSH Protocols; 2006; doi:10.1101/pdb.prot4535 [Abstract] [Full text]