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Mutagenesis by PCR

Protocols 1-10 of 10 total displayed.

Most recent content (1 Mar 2008)
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Streamlined Gene Assembly PCR
Wayne M. Barnes and Elaine R. Frawley
CSH Protocols; 2008; doi:10.1101/pdb.prot4862 [Abstract] [Full text]

Past content (since May 2006)
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PCR Mutagenesis by Overlap Extension and Gene SOE
Abbe N. Vallejo, Robert J. Pogulis, and Larry R. Pease
CSH Protocols; 2008; doi:10.1101/pdb.prot4861 [Abstract] [Full text]

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Setting Up a PCR Laboratory
Theodore E. Mifflin
CSH Protocols; 2007; doi:10.1101/pdb.top14 [Abstract] [Full text]

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Rapid and Efficient Site-directed Mutagenesis by the Single-tube Megaprimer PCR Method
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3467 [Extract] [Full text]

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Site-specific Mutagenesis by Overlap Extension
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3468 [Extract] [Full text]

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In Vitro Mutagenesis Using Double-stranded DNA Templates: Selection of Mutants with DpnI
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3813 [Extract] [Full text]

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Genetic Engineering with PCR
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3836 [Extract] [Full text]

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Mutagenic PCR
R. Craig Cadwell and Gerald F. Joyce
CSH Protocols; 2006; doi:10.1101/pdb.prot4143 [Extract] [Full text]

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Rapid PCR Site-Directed Mutagenesis
Gina L. Costa and Michael P. Weiner
CSH Protocols; 2006; doi:10.1101/pdb.prot4144 [Extract] [Full text]

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PCR-Mediated Gene Disruption: One-Step Method
David C. Amberg, Daniel J. Burke, and Jeffrey N. Strathern
CSH Protocols; 2006; doi:10.1101/pdb.prot4169 [Extract] [Full text]