Mutagenesis

Mouse Mutagenesis Using N-Ethyl-N-Nitrosourea (ENU)
Andrew P. Salinger and Monica J. Justice
CSH Protocols; 2008; doi:10.1101/pdb.prot4985 [Abstract] [Full text]

PCR Mutagenesis by Overlap Extension and Gene SOE
Abbe N. Vallejo, Robert J. Pogulis, and Larry R. Pease
CSH Protocols; 2008; doi:10.1101/pdb.prot4861 [Abstract] [Full text]

Conditional Mutagenesis of the Genome Using Site-Specific DNA Recombination
Kazuaki Ohtsubo and Jamey D. Marth
CSH Protocols; 2007; doi:10.1101/pdb.top12 [Abstract] [Full text]

Genome Modification by Inclusion of loxP Transgene Sequences
Kazuaki Ohtsubo and Jamey D. Marth
CSH Protocols; 2007; doi:10.1101/pdb.prot4761 [Abstract] [Full text]

Cre Recombinase Gene Transfer In Vitro and Detection of loxP-Dependent Recombination
Kazuaki Ohtsubo and Jamey D. Marth
CSH Protocols; 2007; doi:10.1101/pdb.prot4762 [Abstract] [Full text]

Rapid and Efficient Site-directed Mutagenesis by the Single-tube Megaprimer PCR Method
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3467 [Extract] [Full text]

Site-specific Mutagenesis by Overlap Extension
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3468 [Extract] [Full text]

Oligonucleotide-directed Mutagenesis by Elimination of a Unique Restriction Site (USE Mutagenesis)
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3742 [Extract] [Full text]

Preparation of Uracil-containing Single-stranded Bacteriophage M13 DNA
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3811 [Extract] [Full text]

Oligonucleotide-directed Mutagenesis of Single-stranded DNA
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3817 [Extract] [Full text]