Multi-Dimensional Chromatography

Analysis of Complex Protein Mixtures Using Multidimensional Protein Identification Technology (MuDPIT)
David M. Schieltz and Michael P. Washburn
CSH Protocols; 2006; doi:10.1101/pdb.prot4555 [Abstract] [Full text]

Digestion of Insoluble Protein Fractions for Multidimensional Protein Identification Technology (MuDPIT) Analysis
David M. Schieltz and Michael P. Washburn
CSH Protocols; 2006; doi:10.1101/pdb.prot4556 [Abstract] [Full text]

Preparation of Eukaryotic Lysates for 2D Gel Electrophoresis
Hong Ji
CSH Protocols; 2006; doi:10.1101/pdb.prot4571 [Abstract] [Full text]

Radioisotopic Labeling of Eukaryotic Cell Proteins Using ³⁵S
Hong Ji
CSH Protocols; 2006; doi:10.1101/pdb.prot4572 [Abstract] [Full text]

Radioisotopic Labeling of Eukaryotic Cell Proteins Using ³²P
Hong Ji
CSH Protocols; 2006; doi:10.1101/pdb.prot4573 [Abstract] [Full text]

Preparative 2D Gel Electrophoresis with Immobilized pH Gradients: Rehydration of IPG Strips for Isoelectric Focusing of Proteins
Wayne R. Stochaj, Tom Berkelman, and Nancy Laird
CSH Protocols; 2006; doi:10.1101/pdb.prot4574 [Abstract] [Full text]

Preparative 2D Gel Electrophoresis with Immobilized pH Gradients: Isoelectric Focusing of Proteins in a Multipurpose Flatbed Electrophoresis Unit
Wayne R. Stochaj, Tom Berkelman, and Nancy Laird
CSH Protocols; 2006; doi:10.1101/pdb.prot4575 [Abstract] [Full text]

Preparative 2D Gel Electrophoresis with Immobilized pH Gradients: IEF of Proteins in an IEF-Dedicated Electrophoresis Unit
Wayne R. Stochaj, Tom Berkelman, and Nancy Laird
CSH Protocols; 2006; doi:10.1101/pdb.prot4576 [Abstract] [Full text]

Preparation of Vertical SDS Slab Gels: Casting a Single Homogeneous Gel
Wayne R. Stochaj, Tom Berkelman, and Nancy Laird
CSH Protocols; 2006; doi:10.1101/pdb.prot4582 [Abstract] [Full text]

Preparation of Vertical SDS Slab Gels: Simultaneous Casting of Multiple-Gradient Gels
Wayne R. Stochaj, Tom Berkelman, and Nancy Laird
CSH Protocols; 2006; doi:10.1101/pdb.prot4583 [Abstract] [Full text]