cDNA

Maize Tissue Preparation and Extraction of RNA from Target Cells for Genotyping
Kazuhiro Ohtsu and Patrick S. Schnable
CSH Protocols; 2007; doi:10.1101/pdb.prot4784 [Abstract] [Full text]

T7-Based RNA Amplification for Genotyping from Maize Shoot Apical Meristem
Kazuhiro Ohtsu and Patrick S. Schnable
CSH Protocols; 2007; doi:10.1101/pdb.prot4785 [Abstract] [Full text]

SNP Mining from Maize 454 EST Sequences
W. Brad Barbazuk, Scott Emrich, and Patrick S. Schnable
CSH Protocols; 2007; doi:10.1101/pdb.prot4786 [Abstract] [Full text]

Construction of cDNA Libraries • Stage 4: Attachment of Linkers or Adaptors
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3310 [Extract] [Full text]

Construction of cDNA Libraries • Stage 2: Second-strand Synthesis
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3560 [Extract] [Full text]

Construction of cDNA Libraries • Stage 5: Fractionation of cDNA by Gel Filtration through Sepharose CL-4B
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3730 [Extract] [Full text]

Blunt-end Cloning of PCR Products
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3830 [Extract] [Full text]

Amplification of cDNA Generated by Reverse Transcription of mRNA
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3837 [Extract] [Full text]

Radiolabeling of Subtracted cDNA Probes by Random Oligonucleotide Extension
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3860 [Extract] [Full text]

Rapid Amplification of 3' cDNA Ends (3'-RACE)
Joseph Sambrook and David W. Russell
CSH Protocols; 2006; doi:10.1101/pdb.prot3865 [Extract] [Full text]