CSH Protocols, Table of Contents
Volume 2, Issue 7

July 2007

Cover Illustration: Retinal section from an embryo (4 d post-fertilization) stained with Zpr-1, a cone cell marker (blue), and PKC, a bipolar cell marker (red). Image from Immunohistochemistry on Cryosections from Embryonic and Adult Zebrafish Eyes (doi:10.1101/pdb.prot4779).

Protocols

Staining Proteins in Gels with Silver Nitrate
Richard J. Simpson
CSH Protocols; 2007; doi:10.1101/pdb.prot4727
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Isotope-Coded Affinity Tagging of Proteins
Ruedi Aebersold, Timothy J. Griffin, and Sam Donohoe
CSH Protocols; 2007; doi:10.1101/pdb.prot4728
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Genome Modification by Inclusion of loxP Transgene Sequences
Kazuaki Ohtsubo and Jamey D. Marth
CSH Protocols; 2007; doi:10.1101/pdb.prot4761
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Cre Recombinase Gene Transfer In Vitro and Detection of loxP-Dependent Recombination
Kazuaki Ohtsubo and Jamey D. Marth
CSH Protocols; 2007; doi:10.1101/pdb.prot4762
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Long-Term Two-Photon Transcranial Imaging of Synaptic Structures in the Living Brain
Jaime Grutzendler and Wen-Biao Gan
CSH Protocols; 2007; doi:10.1101/pdb.prot4766
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Immunohistochemistry on Cryosections from Embryonic and Adult Zebrafish Eyes
Rosa A. Uribe and Jeffrey M. Gross
CSH Protocols; 2007; doi:10.1101/pdb.prot4779
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Imaging Retinotectal Synaptic Connectivity
Susana Cohen-Cory
CSH Protocols; 2007; doi:10.1101/pdb.prot4782
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Maize Tissue Preparation and Extraction of RNA from Target Cells for Genotyping
Kazuhiro Ohtsu and Patrick S. Schnable
CSH Protocols; 2007; doi:10.1101/pdb.prot4784
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T7-Based RNA Amplification for Genotyping from Maize Shoot Apical Meristem
Kazuhiro Ohtsu and Patrick S. Schnable
CSH Protocols; 2007; doi:10.1101/pdb.prot4785
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SNP Mining from Maize 454 EST Sequences
W. Brad Barbazuk, Scott Emrich and Patrick S. Schnable
CSH Protocols; 2007; doi:10.1101/pdb.prot4786
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Differential Interference Contrast (DIC) Imaging of Living Cells
Noam E. Ziv and Jackie Schiller
CSH Protocols; 2007; doi:10.1101/pdb.prot4787
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In Vivo Electroporation of Neurons
Stephen R. Price
CSH Protocols; 2007; doi:10.1101/pdb.prot4788
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Single-Neuron Labeling Using the Genetic MARCM Method
Liqun Luo
CSH Protocols; 2007; doi:10.1101/pdb.prot4789
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High-Resolution, Intravital 4D Confocal Time-Lapse Imaging in Avian Embryos Using a Teflon Culture Chamber Design
Paul A. Rupp and Paul M. Kulesa
CSH Protocols; 2007; doi:10.1101/pdb.prot4790
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Sagittal Explant Culture for 3D Confocal Time-Lapse Analysis of Chick Peripheral Nervous System Formation
Jennifer C. Kasemeier-Kulesa, Frances Lefcort and Paul M. Kulesa
CSH Protocols; 2007; doi:10.1101/pdb.prot4791
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Construction of a Heated Incubation Chamber around a Microscope Stage for Time-Lapse Imaging
Paul M. Kulesa and Jennifer C. Kasemeier-Kulesa
CSH Protocols; 2007; doi:10.1101/pdb.prot4792
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Information Panels

Inorganic Caged Compounds: Uncaging with Visible Light
Leonardo Zayat, Luis Baraldo and Roberto Etchenique
CSH Protocols; 2007; doi:10.1101/pdb.ip39
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Recommended Steps for a FASTA Search
David W. Mount
CSH Protocols; 2007; doi:10.1101/pdb.ip40
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Steps Used by the BLAST AlgorithmSteps Used by the BLAST Algorithm
David W. Mount
CSH Protocols; 2007; doi:10.1101/pdb.ip41
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Topic Introductions

Conditional Mutagenesis of the Genome Using Site-Specific DNA Recombination
Kazuaki Ohtsubo and Jamey D. Marth
CSH Protocols; 2007; doi:10.1101/pdb.top12
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Setting Up a PCR Laboratory
Theodore E. Mifflin
CSH Protocols; 2007; doi:10.1101/pdb.top14
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Strategies for Sequence Similarity Database Searches
David W. Mount
CSH Protocols; 2007; doi:10.1101/pdb.top15
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Using a FASTA Sequence Database Similarity Search
David W. Mount
CSH Protocols; 2007; doi:10.1101/pdb.top16
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Using the Basic Local Alignment Search Tool (BLAST)
David W. Mount
CSH Protocols; 2007; doi:10.1101/pdb.top17
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