CSH Protocols, Table of Contents
Volume 3, Issue 6

June 2008

Cover Illustration: Surface view of a syncytial stage Drosophila melanogaster embryo double-stained with antisense RNA probes for the CG1962 (green) and canoe (red) mRNAs, which localize to replicating centrosomes and cell junction networks, respectively. Nuclei are counterstained in blue with DAPI. (Image courtesy of Eric Lécuyer and Henry Krause.) For more information on this method, see High-Resolution Fluorescent In Situ Hybridization of Drosophila Embryos and Tissues (doi:10.1101/pdb.prot5019).

Protocols

Reduction of Contamination of Epithelial Cultures by Fibroblasts
Jonathan C.R. Jones
CSH Protocols; 2008; doi:10.1101/pdb.prot4478
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In-Gel S-Pyridylethylation of Gel-Resolved Proteins: Whole Gel Method
Richard J. Simpson
CSH Protocols; 2008; doi:10.1101/pdb.prot4604
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In-Gel S-Pyridylethylation of Gel-Resolved Proteins: Individual Spot Method
Richard J. Simpson
CSH Protocols; 2008; doi:10.1101/pdb.prot4605
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Phenotypic Analysis of Arabidopsis Mutants: Auxin Hormone Response
Mark Estelle
CSH Protocols; 2008; doi:10.1101/pdb.prot4965
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Phenotypic Analysis of Arabidopsis Mutants: Ethylene Hormone Response
Paul B. Larsen and Caren Chang
CSH Protocols; 2008; doi:10.1101/pdb.prot4966
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Phenotypic Analysis of Arabidopsis Mutants: Brassinosteroid Hormone Response
Zhiyong Wang
CSH Protocols; 2008; doi:10.1101/pdb.prot4967
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Isolation and Whole-Cell Patch Clamping of Arabidopsis Guard Cell Protoplasts
Wei Zhang, Sarah E. Nilson, and Sarah M. Assmann
CSH Protocols; 2008; doi:10.1101/pdb.prot5014
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Immunogold Staining of Epoxy Resin Sections for Transmission Electron Microscopy (TEM)
Jeremy N. Skepper and Janet M. Powell
CSH Protocols; 2008; doi:10.1101/pdb.prot5015
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Immunogold Staining of London Resin (LR) White Sections for Transmission Electron Microscopy (TEM)
Jeremy N. Skepper and Janet M. Powell
CSH Protocols; 2008; doi:10.1101/pdb.prot5016
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Immunogold Staining Following Freeze Substitution and Low Temperature Embedding after Chemical Fixation or after Cryoimmobilization for Transmission Electron Microscopy (TEM)
Jeremy N. Skepper and Janet M. Powell
CSH Protocols; 2008; doi:10.1101/pdb.prot5017
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Immunogold Staining of Ultrathin Thawed Cryosections for Transmission Electron Microscopy (TEM)
Jeremy N. Skepper and Janet M. Powell
CSH Protocols; 2008; doi:10.1101/pdb.prot5018
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High-Resolution Fluorescent In Situ Hybridization of Drosophila Embryos and Tissues
Eric Lécuyer, Aleksandar S. Nećakov, Lucía Cáceres, and Henry M. Krause
CSH Protocols; 2008; doi:10.1101/pdb.prot5019
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Information Panels

A Test of the Markov Model of Evolution in Proteins
David W. Mount
CSH Protocols; 2008; doi:10.1101/pdb.ip58
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Comparison of the PAM and BLOSUM Amino Acid Substitution Matrices
David W. Mount
CSH Protocols; 2008; doi:10.1101/pdb.ip59
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Studies of Varying Alignment Algorithm, Amino Acid Scoring Matrix, and Gap Penalties
David W. Mount
CSH Protocols; 2008; doi:10.1101/pdb.ip60
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Topic Introductions

Using PAM Matrices in Sequence Alignments
David W. Mount
CSH Protocols; 2008; doi:10.1101/pdb.top38
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Using BLOSUM in Sequence Alignments
David W. Mount
CSH Protocols; 2008; doi:10.1101/pdb.top39
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Using Gaps and Gap Penalties to Optimize Pairwise Sequence Alignments
David W. Mount
CSH Protocols; 2008; doi:10.1101/pdb.top40
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Copy Number Variation Detection via High-Density SNP Genotyping
Kai Wang and Maja Bucan
CSH Protocols; 2008; doi:10.1101/pdb.top46
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Ultrastructural Immunochemistry
Jeremy N. Skepper and Janet M. Powell
CSH Protocols; 2008; doi:10.1101/pdb.top47
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