CSH Protocols, Table of Contents
Volume 3, Issue 5

May 2008

Cover Illustration: Spread polytene chromosomes of salivary glands from a Drosophila larva were triple-stained with fluorescently tagged antibodies to Heat Shock Transcription Factor (red), RNA Polymerase II (green), and P-TEFb kinase (blue). The larva was heat shock induced for 2.5 min. (Image courtesy of John T. Lis and Janis Werner.) For more information on these methods, see Dissection of Larval Salivary Glands and Polytene Chromosome Preparation (doi:10.1101/pdb.prot4708) and Mapping Protein Distributions on Polytene Chromosomes by Immunostaining (doi:10.1101/pdb.prot4714).

Protocols

Alkaline Phosphatase Treatment of Phosphopeptides: In-Solution Dephosphorylation prior to MALDI-MS Analysis
Hanno Steen, Allan Stensballe, and Ole N. Jensen
CSH Protocols; 2008; doi:10.1101/pdb.prot4610
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Alkaline Phosphatase Treatment of Phosphopeptides: In-Solution Dephosphorylation after MALDI-MS Analysis
Hanno Steen, Allan Stensballe, and Ole N. Jensen
CSH Protocols; 2008; doi:10.1101/pdb.prot4611
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Alkaline Phosphatase Treatment of Phosphopeptides: On-Probe Dephosphorylation after MALDI-MS Analysis
Hanno Steen, Allan Stensballe, and Ole N. Jensen
CSH Protocols; 2008; doi:10.1101/pdb.prot4612
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Karyotyping Mouse Cells
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols; 2008; doi:10.1101/pdb.prot4706
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Dissection of Larval Salivary Glands and Polytene Chromosome Preparation
James A. Kennison
CSH Protocols; 2008; doi:10.1101/pdb.prot4708
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Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
Renato Paro
CSH Protocols; 2008; doi:10.1101/pdb.prot4714
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Phenotypic Analysis of Arabidopsis Mutants: Gibberellin/Abscisic Acid/Paclobutrazol Hormone Response
Miguel Blázquez
CSH Protocols; 2008; doi:10.1101/pdb.prot4964
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Hematoxylin and Eosin Staining of Tissue and Cell Sections
Andrew H. Fischer, Kenneth A. Jacobson, Jack Rose, and Rolf Zeller
CSH Protocols; 2008; doi:10.1101/pdb.prot4986
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Cutting Sections of Paraffin-Embedded Tissues
Andrew H. Fischer, Kenneth A. Jacobson, Jack Rose, and Rolf Zeller
CSH Protocols; 2008; doi:10.1101/pdb.prot4987
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Preparation of Slides and Coverslips for Microscopy
Andrew H. Fischer, Kenneth A. Jacobson, Jack Rose, and Rolf Zeller
CSH Protocols; 2008; doi:10.1101/pdb.prot4988
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Paraffin Embedding Tissue Samples for Sectioning
Andrew H. Fischer, Kenneth A. Jacobson, Jack Rose, and Rolf Zeller
CSH Protocols; 2008; doi:10.1101/pdb.prot4989
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Decalcifying Tissues for Paraffin Embedding
Andrew H. Fischer, Kenneth A. Jacobson, Jack Rose, and Rolf Zeller
CSH Protocols; 2008; doi:10.1101/pdb.prot4990
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Whole Genome Amplification by T7-Based Linear Amplification of DNA (TLAD): I. CIP Treatment of Samples and Tailing Reaction with Terminal Transferase
Chih Long Liu, Bradley E. Bernstein, and Stuart L. Schreiber
CSH Protocols; 2008; doi:10.1101/pdb.prot5002
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Whole Genome Amplification by T7-Based Linear Amplification of DNA (TLAD): II. Second-Strand Synthesis and In Vitro Transcription
Chih Long Liu, Bradley E. Bernstein, and Stuart L. Schreiber
CSH Protocols; 2008; doi:10.1101/pdb.prot5003
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Whole Genome Amplification by T7-Based Linear Amplification of DNA (TLAD): III. Sample Purification
Chih Long Liu, Bradley E. Bernstein, and Stuart L. Schreiber
CSH Protocols; 2008; doi:10.1101/pdb.prot5004
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Information Panels

Making Solutions from Dry Chemicals
Dany Spencer Adams
CSH Protocols; 2008; doi:10.1101/pdb.ip53
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Making Solutions from Hydrated Compounds
Dany Spencer Adams
CSH Protocols; 2008; doi:10.1101/pdb.ip54
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Making and Diluting Stock Solutions
Dany Spencer Adams
CSH Protocols; 2008; doi:10.1101/pdb.ip55
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Topic Introductions

Fixation and Permeabilization of Cells and Tissues
Andrew H. Fischer, Kenneth A. Jacobson, Jack Rose, and Rolf Zeller
CSH Protocols; 2008; doi:10.1101/pdb.top36
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Whole Genome Amplification by T7-Based Linear Amplification of DNA (TLAD): Overview
Chih Long Liu, Bradley E. Bernstein, and Stuart L. Schreiber
CSH Protocols; 2008; doi:10.1101/pdb.top42
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