CSH Protocols, Table of Contents
Volume 2, Issue 4

April 2007

Cover Illustration: Confocal time-lapse microscopy of a microinjected Drosophila embryo. Image from Quantitative Microinjection of Drosophila Embryos (doi:10.1101/pdb.prot4718).

Protocols

Protein Interactions Captured by Chemical Cross-linking: Simple Cross-linking Screen Using Sulfo-MBS
Owen W. Nadeau, Gerald M. Carlson
CSH Protocols; 2007; doi:10.1101/pdb.prot4587
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Protein Interactions Captured by Chemical Cross-linking: One-step Cross-linking with Formaldehyde
Owen W. Nadeau, Gerald M. Carlson
CSH Protocols; 2007; doi:10.1101/pdb.prot4634
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Protein Interactions Captured by Chemical Cross-linking: Two-step Cross-linking with ANB•NOS
Owen W. Nadeau, Gerald M. Carlson
CSH Protocols; 2007; doi:10.1101/pdb.prot4635
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Using Genetically Engineered Kinases to Screen for Novel Protein Kinase Substrates: Identification of a Mutant Kinase/ATP Analog Pair
Scott T. Eblen, N. Vinay Kumar, Michael J. Weber
CSH Protocols; 2007; doi:10.1101/pdb.prot4636
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Using Genetically Engineered Kinases to Screen for Novel Protein Kinase Substrates: Generation of [-³²P]ATP Analog from ADP Analog?
Scott T. Eblen, N. Vinay Kumar, Michael J. Weber
CSH Protocols; 2007; doi:10.1101/pdb.prot4637
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Using Genetically Engineered Kinases to Screen for Novel Protein Kinase Substrates: Phosphorylation of Kinase-Associated Substrates
Scott T. Eblen, N. Vinay Kumar, Michael J. Weber
CSH Protocols; 2007; doi:10.1101/pdb.prot4638
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Using Genetically Engineered Kinases to Screen for Novel Protein Kinase Substrates: Phosphorylation of Substrates in Cell Lysates with Exogenous Kinase
Scott T. Eblen, N. Vinay Kumar, Michael J. Weber
CSH Protocols; 2007; doi:10.1101/pdb.prot4639
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Screening Kinase Phosphorylation Motifs Using Peptide Libraries
Isaac A. Manke, Michael B. Yaffe
CSH Protocols; 2007; doi:10.1101/pdb.prot4640
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Zinc/Imidazole Procedure for Visualization of Proteins in Gels by Negative Staining
Richard J. Simpson
CSH Protocols; 2007; doi:10.1101/pdb.prot4701
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Dissection Techniques for Pupal and Larval Drosophila Eyes
Tanya Wolff
CSH Protocols; 2007; doi:10.1101/pdb.prot4715
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Staining of Drosophila Tissues with Antibodies
Tanya Wolff
CSH Protocols; 2007; doi:10.1101/pdb.prot4716
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Collection, Dechorionation, and Preparation of Drosophila Embryos for Quantitative Microinjection
Daniel P. Kiehart, Janice M. Crawford, Ruth A Montague
CSH Protocols; 2007; doi:10.1101/pdb.prot4717
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Quantitative Microinjection of Drosophila Embryos
Daniel P. Kiehart, Janice M. Crawford, Ruth A Montague
CSH Protocols; 2007; doi:10.1101/pdb.prot4718
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Staining Proteins in Gels with Coomassie Blue
Richard J. Simpson
CSH Protocols; 2007; doi:10.1101/pdb.prot4719
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Imaging X-gal-stained Mouse Embryos
Akio Kobayashi
CSH Protocols; 2007; doi:10.1101/pdb.prot4724
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Staining Whole Mouse Embryos For ß-Galactosidase (lacZ) Activity
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, Richard Behringer
CSH Protocols; 2007; doi:10.1101/pdb.prot4725
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Staining Frozen Mouse Embryo Sections for ß-Galactosidase (lacZ) Activity
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, Richard Behringer
CSH Protocols; 2007; doi:10.1101/pdb.prot4726
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Preparation of GST Fusion Proteins
Margret B. Einarson, Elena N. Pugacheva, Jason R.Orlinick
CSH Protocols; 2007; doi:10.1101/pdb.prot4738
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cAMP Assays
Gouzel Karimova, Daniel Ladant
CSH Protocols; 2007; doi:10.1101/pdb.prot4739
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Information Panels

Quenching of Protein Cross-linking
Owen W. Nadeau, Gerald M. Carlson
CSH Protocols; 2007; doi:10.1101/pdb.ip12
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Nomenclature on Proteases, Proteinases, and Peptidases
Richard J. Simpson
CSH Protocols; 2007; doi:10.1101/pdb.ip13
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Topic Introductions

Quantitative Microinjection of Drosophila Embryos: General Strategy
Daniel P. Kiehart, Janice M. Crawford, Ruth A Montague
CSH Protocols; 2007; doi:10.1101/pdb.top5
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