CSH Protocols, Table of Contents
Volume 3, Issue 3

March 2008

Cover Illustration: Neural crest cells from rhombomeres 3 to 5 (r3-r5) and post-otic segments fluorescently labeled (green) at HH Stage 8 with the photoactivatable fluorescent protein, KikGR, invade the second, third, and fourth branchial arches in a chick embryo. Early emerging r4 neural crest cells (red), selectively photoconverted by 40-nm confocal laser light, populate the distal portion of the second branchial arch. (Image courtesy of Danny Stark and Paul Kulesa.) For more information on this method, see Photoactivation Cell Labeling for Cell Tracing in Avian Development (doi:10.1101/pdb.prot4975).

Protocols

Streamlined Gene Assembly PCR
Wayne M. Barnes and Elaine R. Frawley
CSH Protocols; 2008; doi:10.1101/pdb.prot4862
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Cell-Penetrating Peptide-Mediated Delivery of Peptide Nucleic Acid (PNA) Oligomers
Pontus Lundberg, Kalle Kilk, and Ülo Langel
CSH Protocols; 2008; doi:10.1101/pdb.prot4889
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Labeling Lysosomes in Live Cells with Fluorescent Dyes for Imaging
Brad Chazotte
CSH Protocols; 2008; doi:10.1101/pdb.prot4929
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Vital Stain for Plant Cytoplasm
Birgit Schwab and Martin Hülskamp
CSH Protocols; 2008; doi:10.1101/pdb.prot4936
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Preparation of Arabidopsis Tissue Sections of Fixed Material
Stuart Baum
CSH Protocols; 2008; doi:10.1101/pdb.prot4955
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Phenotypic Analysis of Arabidopsis Mutants: Quantitative Analysis of Root Growth
Peter Doerner
CSH Protocols; 2008; doi:10.1101/pdb.prot4960
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Computer-Based Analysis of the Optokinetic Response in Zebrafish Larvae
Corinne Hodel and Stephan C.F. Neuhauss
CSH Protocols; 2008; doi:10.1101/pdb.prot4961
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Phenotypic Analysis of Arabidopsis Mutants: Hypocotyl Length
Justin Borevitz and Michael Neff
CSH Protocols; 2008; doi:10.1101/pdb.prot4962
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DNA Immunoprecipitation (DIP) for the Determination of DNA-Binding Specificity
Andrea J. Gossett and Jason D. Lieb
CSH Protocols; 2008; doi:10.1101/pdb.prot4972
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Electroretinogram (ERG) Measurements in Larval Zebrafish
Valerie C. Fleisch, Tiziana Jametti, and Stephan C.F. Neuhauss
CSH Protocols; 2008; doi:10.1101/pdb.prot4973
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Methylated CpG Island Amplification and Microarray (MCAM) for High-Throughput Analysis of DNA Methylation
Marcos R.H. Estécio, Pearlly S. Yan, Tim H-M. Huang, and Jean-Pierre J. Issa
CSH Protocols; 2008; doi:10.1101/pdb.prot4974
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Photoactivation Cell Labeling for Cell Tracing in Avian Development
Danny A. Stark, Jennifer C. Kasemeier-Kulesa, and Paul M. Kulesa
CSH Protocols; 2008; doi:10.1101/pdb.prot4975
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Culture of HEK293-EBNA1 Cells for Production of Recombinant Proteins
Roseanne Tom, Louis Bisson, and Yves Durocher
CSH Protocols; 2008; doi:10.1101/pdb.prot4976
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Transfection of HEK293-EBNA1 Cells in Suspension with Linear PEI for Production of Recombinant Proteins
Roseanne Tom, Louis Bisson, and Yves Durocher
CSH Protocols; 2008; doi:10.1101/pdb.prot4977
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Transfection of Adherent HEK293-EBNA1 Cells in a Six-Well Plate with Branched PEI for Production of Recombinant Proteins
Roseanne Tom, Louis Bisson, and Yves Durocher
CSH Protocols; 2008; doi:10.1101/pdb.prot4978
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Transfection of HEK293-EBNA1 Cells in Suspension with 293fectin for Production of Recombinant Proteins
Roseanne Tom, Louis Bisson, and Yves Durocher
CSH Protocols; 2008; doi:10.1101/pdb.prot4979
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Purification of His-Tagged Proteins Using Fractogel-Cobalt
Roseanne Tom, Louis Bisson, and Yves Durocher
CSH Protocols; 2008; doi:10.1101/pdb.prot4980
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Information Panels

Media for Mounting Fixed Cells on Microscope Slides
Andrew H. Fischer, Kenneth A. Jacobson, Jack Rose, and Rolf Zeller
CSH Protocols; 2008; doi:10.1101/pdb.ip52
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Topic Introductions

Strategies for Overcoming PCR Inhibition
Peter Rådström, Charlotta Löfström, Maria Lövenklev, Rickard Knutsson, and Petra Wolffs
CSH Protocols; 2008; doi:10.1101/pdb.top20
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Genetic Analysis of Arabidopsis Mutants
Detlef Weigel and Jane Glazebrook
CSH Protocols; 2008; doi:10.1101/pdb.top35
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