CSH Protocols, Table of Contents
Volume 2, Issue 2

February 2007

Cover Illustration: Triple staining of chromosomes, centromeres, and microtubules in a living HeLa cell. Image from Imaging Hoechst-Labeled Chromosomes and Fluorescent Proteins during the Cell Cycle (doi:10.1101/pdb.prot4673).

Protocols

Reduction and S-Carboxymethylation of Proteins: Microscale Method
Richard J. Simpson
CSH Protocols; 2007; doi:10.1101/pdb.prot4568
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Reduction and S-Carboxymethylation of Proteins: Large-Scale Method
Richard J. Simpson
CSH Protocols; 2007; doi:10.1101/pdb.prot4569
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In Situ SDS-PAGE Peptide-Mapping Procedure (Cleveland Method)
Richard J. Simpson
CSH Protocols; 2007; doi:10.1101/pdb.prot4570
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Configuration, Column Construction, and Column Packing for a Capillary Liquid Chromatography System
Robert L. Moritz
CSH Protocols; 2007; doi:10.1101/pdb.prot4578
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SDS-PAGE Peptide-Mapping Procedure (Cleveland Method)
Richard J. Simpson
CSH Protocols; 2007; doi:10.1101/pdb.prot4591
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Analysis of Proteomes Using the Molecular Scanner
Catherine Déon, Willy Bienvenut, Jean-Charles Sanchez, Denis F. Hochstrasser, Markus Müller, Robin Gras, and Ron D. Appel
CSH Protocols; 2007; doi:10.1101/pdb.prot4592
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Affinity Purification of Interacting Proteins from Cell Lysates
Manuel Baca and Jian-Guo Zhang
CSH Protocols; 2007; doi:10.1101/pdb.prot4593
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Analysis of the Topology of Protein Complexes Using Cross-Linking and Mass Spectrometry
Juri Rappsilber and Matthias Mann
CSH Protocols; 2007; doi:10.1101/pdb.prot4594
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Transfecting Cultured Hippocampal Neurons with an Actin-GFP Plasmid
Andrew Matus, Virginie Biou, Heike Brinkhaus, and Martijn Roelandse
CSH Protocols; 2007; doi:10.1101/pdb.prot4664
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Imaging Hoechst-Labeled Chromosomes and Fluorescent Proteins during the Cell Cycle
Tokuko Haraguchi and Yasushi Hiraoka
CSH Protocols; 2007; doi:10.1101/pdb.prot4673
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Studying Mitosis in Cultured Mammalian Cells
Patricia Wadsworth
CSH Protocols; 2007; doi:10.1101/pdb.prot4674
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Purification of Bovine Lens and Bacterially Expressed Human Vimentin
Edward R. Kuczmarski and Robert D. Goldman
CSH Protocols; 2007; doi:10.1101/pdb.prot4675
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Preparation and Microinjection of x-Rhodamine-Labeled Vimentin
Edward R. Kuczmarski and Robert D. Goldman
CSH Protocols; 2007; doi:10.1101/pdb.prot4676
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Quantitative GUS Activity Assay in Intact Plant Tissue
Miguel Blázquez
CSH Protocols; 2007; doi:10.1101/pdb.prot4688
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Subcellular Localization of GUS- and GFP-Tagged Proteins in Onion Epidermal Cells
Albrecht von Arnim
CSH Protocols; 2007; doi:10.1101/pdb.prot4689
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Quantitative GUS Activity Assay of Plant Extracts
Miguel Blázquez
CSH Protocols; 2007; doi:10.1101/pdb.prot4690
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Transgene Expression in Regenerated Roots
Jocelyn Malamy
CSH Protocols; 2007; doi:10.1101/pdb.prot4691
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Transient Expression in Protoplasts
Fumiaki Katagiri
CSH Protocols; 2007; doi:10.1101/pdb.prot4692
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Information Panels

Imaging Actin in Tissue Slices from Transgenic Mouse Brain
Andrew Matus, Virginie Biou, Heike Brinkhaus, and Martijn Roelandse
CSH Protocols; 2007; doi:10.1101/pdb.ip28
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Live-Cell Imaging of GFP in Plants
Andrew Groover and David Jackson
CSH Protocols; 2007; doi:10.1101/pdb.ip31
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Chambers for Examination of Live Cells under Mechanical Stress
Brian P. Helmke and Peter F. Davies
CSH Protocols; 2007; doi:10.1101/pdb.ip32
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