CSH Protocols, Table of Contents
Volume 2, Issue 12

December 2007

Cover Illustration: HH Stage 3+ quail embryo electroporated with an H2B-GFP construct using the method described in Cui et al., below. Time lapse images were taken every 6 minutes over 28 hours. Yellow indicates the track of the last 30 most recently acquired time points, green indicates the track of the last 3 most recently acquired time points. For more information on this method, see Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian Embryos (doi:10.1101/pdb.prot4894).

Protocols

Generating Imaginal Disc Clones in Drosophila
Seth S. Blair
CSH Protocols; 2007; doi:10.1101/pdb.prot4793
Abstract
Full Text

Dissection of Imaginal Discs in Drosophila
Seth S. Blair
CSH Protocols; 2007; doi:10.1101/pdb.prot4794
Abstract
Full Text

Fixation of Imaginal Discs in Drosophila
Seth S. Blair
CSH Protocols; 2007; doi:10.1101/pdb.prot4795
Abstract
Full Text

Triple-Label Fluorescent Antibody Staining of Imaginal Discs in Drosophila
Seth S. Blair
CSH Protocols; 2007; doi:10.1101/pdb.prot4796
Abstract
Full Text

Nickel-Intensified ABC-HRP Antibody Staining of Imaginal Discs in Drosophila
Seth S. Blair
CSH Protocols; 2007; doi:10.1101/pdb.prot4797
Abstract
Full Text

Testing Spatial and Nonspatial Learning Using a Morris Water Maze
Carol AnnPaul, Barbara Beltz, and Joanne Berger-Sweeney
CSH Protocols; 2007; doi:10.1101/pdb.prot4801
Abstract
Full Text

Hydroxyl-Radical Footprinting
Michael Carey and Stephen T. Smale
CSH Protocols; 2007; doi:10.1101/pdb.prot4810
Abstract
Full Text

Phosphate Ethylation Interference Assay
Michael Carey and Stephen T. Smale
CSH Protocols; 2007; doi:10.1101/pdb.prot4811
Abstract
Full Text

Methylation Interference Assay
Michael Carey and Stephen T. Smale
CSH Protocols; 2007; doi:10.1101/pdb.prot4812
Abstract
Full Text

Chemical Two-Photon Uncaging
Diana L. Pettit and George J. Augustine
CSH Protocols; 2007; doi:10.1101/pdb.prot4850
Abstract
Full Text

Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian Embryos
Cheng Cui, Brenda Rongish, Charles Little and RustyLansford
CSH Protocols; 2007; doi:10.1101/pdb.prot4894
Abstract
Full Text

Preparation of Cells in 96-Well Plates for siRNA Transfection and High-Content Analysis
Elizabeth P. Roquemore
CSH Protocols; 2007; doi:10.1101/pdb.prot4896
Abstract
Full Text

Optimization of siRNA Knockdown in EGFP-Expressing Cell Lines Using High-Content Analysis
Elizabeth P. Roquemore
CSH Protocols; 2007; doi:10.1101/pdb.prot4897
Abstract
Full Text

Analysis of siRNA Knockdown of Cell-Cycle Control Genes in G₁/S and G₂/M Cell-Cycle Phase Marker Cell Lines Using Multiplexed High-Content Analysis
Elizabeth P. Roquemore
CSH Protocols; 2007; doi:10.1101/pdb.prot4898
Abstract
Full Text

Construction of a Single-Axis Molecular Puller for Measuring Polysaccharide and Protein Mechanics by Atomic Force Microscopy
Mahir Rabbi and Piotr E. Marszalek
CSH Protocols; 2007; doi:10.1101/pdb.prot4899
Abstract
Full Text

Measuring Polysaccharide Mechanics by Atomic Force Microscopy
Mahir Rabbi and Piotr E. Marszalek
CSH Protocols; 2007; doi:10.1101/pdb.prot4900
Abstract
Full Text

Measuring Protein Mechanics by Atomic Force Microscopy
Mahir Rabbi and Piotr E. Marszalek
CSH Protocols; 2007; doi:10.1101/pdb.prot4901
Abstract
Full Text

Organotypic Slice Culture of Embryonic Brain Tissue
Ray A.M. Daza, Chris Englund and Robert F. Hevner
CSH Protocols; 2007; doi:10.1101/pdb.prot4914
Abstract
Full Text

Fate-Mapping Technique: Using Carbocyanine Dyes for Vital Labeling of Cells in Gastrula-Stage Mouse Embryos Cultured In Vitro
Heidi Bildsoe, Vanessa Franklin, and Patrick P.L. Tam
CSH Protocols; 2007; doi:10.1101/pdb.prot4915
Abstract
Full Text

Topic Introductions

Dot Matrix Pairwise Sequence Comparison
David W. Mount
CSH Protocols; 2007; doi:10.1101/pdb.top31
Abstract
Full Text