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Cover Illustration: Grafting procedure from Fate-Mapping Technique: Grafting Fluorescent Cells into Gastrula-Stage Mouse Embryos at 7-7.5 Days Post-coitum (doi:10.1101/pdb.prot4892).
Preparation of Reversed-Phase Microcolumns
Martin R. Larsen
CSH Protocols; 2007; doi:10.1101/pdb.prot4606
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Phosphopeptide Purification by IMAC with Fe(III) and Ga(III)
Hanno Steen, Allan Stensballe, and Ole N. Jensen
CSH Protocols; 2007; doi:10.1101/pdb.prot4607
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Preparation and Use of Microcolumns for Sample Desalting or Nanoscale IMAC
Hanno Steen, Allan Stensballe, and Ole N. Jensen
CSH Protocols; 2007; doi:10.1101/pdb.prot4608
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Preparation of Immobilized Enzyme Microcolumns
Martin R. Larsen
CSH Protocols; 2007; doi:10.1101/pdb.prot4609
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Offline Micro-IMAC Enrichment of Phosphoproteins
Garry L. Corthals, Valerie C. Wasinger, and David R. Goodlett
CSH Protocols; 2007; doi:10.1101/pdb.prot4624
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Analysis of Phosphopeptides by µLC-ESI-MS/MS
Garry L. Corthals, Valerie C. Wasinger, and David R. Goodlett
CSH Protocols; 2007; doi:10.1101/pdb.prot4625
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Imaging Mouse Embryos after Whole-Mount In Situ Hybridization
Maki Wakamiya
CSH Protocols; 2007; doi:10.1101/pdb.prot4774
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Sectioning Mouse Embryos after Whole-Mount In Situ Hybridization
Michael Shen
CSH Protocols; 2007; doi:10.1101/pdb.prot4775
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Handling Mouse Blastocysts for Fixation
Andras Nagy, Marina Gertsenstein, Kristina Vintersten and Richard Behringer
CSH Protocols; 2007; doi:10.1101/pdb.prot4814
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Embedding Mouse Embryos and Tissues in Wax
Andras Nagy, Marina Gertsenstein, Kristina Vintersten and Richard Behringer
CSH Protocols; 2007; doi:10.1101/pdb.prot4815
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Preparing Glass Slides and Coverslips for In Situ Hybridization
Andras Nagy, Marina Gertsenstein, Kristina Vintersten and Richard Behringer
CSH Protocols; 2007; doi:10.1101/pdb.prot4817
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Dewaxing and Rehydrating Sections prior to In Situ Hybridization
Andras Nagy, Marina Gertsenstein, Kristina Vintersten and Richard Behringer
CSH Protocols; 2007; doi:10.1101/pdb.prot4818
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In Situ Hybridization of Mouse Embryo and Tissue Sections with Radiolabeled RNA Probes
Andras Nagy, Marina Gertsenstein, Kristina Vintersten and Richard Behringer
CSH Protocols; 2007; doi:10.1101/pdb.prot4821
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In Situ Hybridization of Whole-Mount Mouse Embryos with RNA Probes: Preparation of Embryos and Probes
Thomas Lufkin
CSH Protocols; 2007; doi:10.1101/pdb.prot4822
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In Situ Hybridization of Whole-Mount Mouse Embryos with RNA Probes: Hybridization, Washes, and Histochemistry
Thomas Lufkin
CSH Protocols; 2007; doi:10.1101/pdb.prot4823
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Imaging Real-Time Gene Expression in Mammalian Cells with Single-Transcript Resolution
Amber L. Wells, John S. Condeelis, Robert H. Singer and Daniel Zenklusen
CSH Protocols; 2007; doi:10.1101/pdb.prot4869
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Imaging Real-Time Gene Expression in Living Yeast
Daniel Zenklusen, John S. Condeelis, Robert H. Singer and Amber L. Wells
CSH Protocols; 2007; doi:10.1101/pdb.prot4870
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Imaging Real-Time Gene Expression in Living Systems with Single-Transcript Resolution: Image Analysis of Single mRNA Transcripts
Amber L. Wells, John S. Condeelis, Robert H. Singer and Daniel Zenklusen
CSH Protocols; 2007; doi:10.1101/pdb.prot4871
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Imaging Real-Time Gene Expression in Living Systems with Single-Transcript Resolution: Single mRNA Particle Tracking with ImageJ-Based Analysis
Amber L. Wells, John S. Condeelis, Robert H. Singer and Daniel Zenklusen
CSH Protocols; 2007; doi:10.1101/pdb.prot4872
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Micrococcal Nuclease-Southern Blot Assay: I. MNase and Restriction Digestions
Michael Carey and Stephen T. Smale
CSH Protocols; 2007; doi:10.1101/pdb.prot4890
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Micrococcal Nuclease-Southern Blot Assay: II. Capillary Transfer and Hybridization
Michael Carey and Stephen T. Smale
CSH Protocols; 2007; doi:10.1101/pdb.prot4891
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Fate-Mapping Technique: Grafting Fluorescent Cells into Gastrula-Stage Mouse Embryos at 7-7.5 Days Post-coitum
Vanessa J. Franklin, Heidi Bildsoe and Patrick P.L. Tam
CSH Protocols; 2007; doi:10.1101/pdb.prot4892
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Fate-Mapping Technique: Targeted Whole-Embryo Electroporation of DNA Constructs into the Germ Layers of Mouse Embryos 7-7.5 Days Post-coitum
Poh-Lynn Khoo, Vanessa J. Franklin and Patrick P.L. Tam
CSH Protocols; 2007; doi:10.1101/pdb.prot4893
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Combined Flow Cytometric Measurement of Two Cell-Surface Antigens and DNA-RNA Content
Ingrid Schmid
CSH Protocols; 2007; doi:10.1101/pdb.prot4895
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Quantitation of DNA and RNA
Carlos F. Barbas III, Dennis R. Burton, Jamie K. Scott and Gregg J. Silverman
CSH Protocols; 2007; doi:10.1101/pdb.ip47
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Imaging Real-Time Gene Expression in Living Systems with Single-Transcript Resolution: Construct Design and Imaging System Setup
Amber L. Wells, John S. Condeelis, Robert H. Singer and Daniel Zenklusen
CSH Protocols; 2007; doi:10.1101/pdb.top28
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