CSH Protocols, Table of Contents
Volume 1, Issue 6

November 2006

Cover Illustration: Transgenic L1 C. elegans larva expressing Ce-lamin fused to GFP. Image from Live Imaging of Caenorhabditis elegans: Examples (doi:10.1101/pdb.ip19).

Protocols

Site-Specific Protein-DNA Photo-Cross-Linking: Analysis of Structural Organization of Protein-DNA and Multiprotein-DNA Complexes
Nikolai Naryshkin, Andrey Revyakin, and Richard H. Ebright
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4588
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Split Luciferase Complementation Assay for Studying Interaction of Proteins X and Y in Living Mice
Ramasam Paulmurugan, Pritha Ray, Abhijit De, Carmel T. Chan, and Sanjir Sam Gambhir
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4595
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Split Luciferase Complementation Assay for Studying Interaction of Proteins X and Y in Cells
Ramasam Paulmurugan, Pritha Ray, Abhijit De, Carmel T. Chan, and Sanjir Sam Gambhir
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4596
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Imaging Protein Interactions by FRET Microscopy: FRET Measurements by Sensitized Emission
Peter J. Verveer, Oliver Rocks, Ailsa G. Harpur, and Philippe I.H. Bastiaens
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4597
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Imaging Protein Interactions by FRET Microscopy: FRET Measurements by Acceptor Photobleaching
Peter J. Verveer, Oliver Rocks, Ailsa G. Harpur, and Philippe I.H. Bastiaens
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4598
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Imaging Protein Interactions by FRET Microscopy: FLIM Measurements
Peter J. Verveer, Oliver Rocks, Ailsa G. Harpur, and Philippe I.H. Bastiaens
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4599
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Photoactivation-Based Labeling and In Vivo Tracking of RNA Molecules in the Nucleus
Joan C. Ritland Politz, Richard A. Tuft, and Thoru Pederson
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4600
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Live Imaging of Caenorhabditis elegans: Preparation of Samples
Benjamin Podbilewicz and Yosef Gruenbaum
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4601
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Time-Lapse Cinematography in Living Drosophila Tissues: Preparation of Material
Ilan Davis and Richard M. Parton
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4602
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Imaging of Organelle Membrane Systems and Membrane Traffic in Living Cells
Jennifer Lippincott-Schwartz and Erik Lee Snapp
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4603
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Detection of Ubiquitylated Proteins in Mammalian Cells
William P. Tansey
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4616
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Bradford Assay
Ed Harlow and David Lane
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4644
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Imaging Protein Interactions by FRET Microscopy: Labeling Proteins with Fluorescent Dyes
Peter J. Verveer, Oliver Rocks, Ailsa G. Harpur, and Philippe I.H. Bastiaens
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4645
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Imaging Protein Interactions by FRET Microscopy: Cell Preparation for FRET Analysis
Peter J. Verveer, Oliver Rocks, Ailsa G. Harpur, and Philippe I.H. Bastiaens
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4646
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Identification of Positive GATEWAY Expression Clones When Both the pENTRY and pDEST Vectors Contain the Same Marker for Bacterial Selection
Norlia Basherudin and Mark D. Curtis
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.prot4647
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Information Panel

Measuring FRET by Sensitized Emission
Peter J. Verveer, Oliver Rocks, Ailsa G. Harpur, and Philippe I.H. Bastiaens
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.ip14
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Measuring FRET by Acceptor Photobleaching
Peter J. Verveer, Oliver Rocks, Ailsa G. Harpur, and Philippe I.H. Bastiaens
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.ip15
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An Experimental Setup for Frequency Domain FLIM
Peter J. Verveer, Oliver Rocks, Ailsa G. Harpur, and Philippe I.H. Bastiaens
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.ip16
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FLIM Measurements and Frequency Domain FLIM Data Analysis
Peter J. Verveer, Oliver Rocks, Ailsa G. Harpur, and Philippe I.H. Bastiaens
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.ip17
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Live Imaging of Caenorhabditis elegans: Observation of Nematodes and Data Collection
Benjamin Podbilewicz and Yosef Gruenbaum
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.ip18
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Live Imaging of Caenorhabditis elegans: Examples
Benjamin Podbilewicz and Yosef Gruenbaum
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.ip19
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Fluorescent Reagents for Live Cell Imaging and Their Introduction into Cells
Ilan Davis and Richard M. Parton
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.ip20
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Selection of Appropriate Imaging Equipment and Methodology for Live Cell Imaging in Drosophila
Ilan Davis and Richard M. Parton
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.ip21
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Membrane Trafficking and Organelle Reagents
Jennifer Lippincott-Schwartz and Erik Lee Snapp
Cold Spring Harbor Protocols 2006; doi:10.1101/pdb.ip23
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