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Cover Illustration: Simultaneous imaging of Nomarski (blue) and eff-1promoter::GFP (green) in developing C. elegans embryos reveals timing of cell fusion. Image from Live Imaging of Caenorhabditis elegans (doi:10.1101/pdb.ip19).
Western Analysis Using the Chemiluminescent Alkaline Phosphatase Substrate CSPD
Peter Gillespie
CSH Protocols; 2006; doi:10.1101/pdb.prot4256
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Staining Immunoblots for Total Protein Using Ponceau S
Ed Harlow and David Lane
CSH Protocols; 2006; doi:10.1101/pdb.prot4269
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Staining Immunoblots for Total Protein Using India Ink
Ed Harlow and David Lane
CSH Protocols; 2006; doi:10.1101/pdb.prot4270
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Immunoblotting: Antigen Detection Using Chemiluminescence
Ed Harlow and David Lane
CSH Protocols; 2006; doi:10.1101/pdb.prot4271
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Immunoblotting: Antigen Detection Using Chromogenic Methods
Ed Harlow and David Lane
CSH Protocols; 2006; doi:10.1101/pdb.prot4272
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Stripping Immunoblots for Reprobing or Storage
Ed Harlow and David Lane
CSH Protocols; 2006; doi:10.1101/pdb.prot4273
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Immunoaffinity Purification: Binding of Antigen to Antibody-Bead Matrix in a Column
Ed Harlow and David Lane
CSH Protocols; 2006; doi:10.1101/pdb.prot4274
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Immunoaffinity Purification: Binding of Antigen to Antibody-Bead Matrix in a Suspension
Ed Harlow and David Lane
CSH Protocols; 2006; doi:10.1101/pdb.prot4275
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Immunoaffinity Purification: Eluting Antigens from Immunoaffinity Columns
Ed Harlow and David Lane
CSH Protocols; 2006; doi:10.1101/pdb.prot4276
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Storage of Purified Antibodies
Ed Harlow and David Lane
CSH Protocols; 2006; doi:10.1101/pdb.prot4282
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Antibody Purification on Protein A or Protein G Columns
Ed Harlow and David Lane
CSH Protocols; 2006; doi:10.1101/pdb.prot4283
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Purification of Antibodies on an Antigen Column
Ed Harlow and David Lane
CSH Protocols; 2006; doi:10.1101/pdb.prot4284
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Immunoblotting: Semi-Dry Electrophoretic Transfer of Proteins from Gels to Membranes
Ed Harlow and David Lane
CSH Protocols; 2006; doi:10.1101/pdb.prot4301
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Immunoblotting: Submerged Electrophoretic Transfer of Proteins from Gels to Membranes
Ed Harlow and David Lane
CSH Protocols; 2006; doi:10.1101/pdb.prot4302
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Immunoaffinity Purification: Coupling Antibodies to Protein A or G Bead Columns
Ed Harlow and David Lane
CSH Protocols; 2006; doi:10.1101/pdb.prot4303
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Immunoaffinity Purification: Coupling Antibodies to Activated Beads
Ed Harlow and David Lane
CSH Protocols; 2006; doi:10.1101/pdb.prot4304
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Preparing Acetone Powders
Ed Harlow and David Lane
CSH Protocols; 2006; doi:10.1101/pdb.prot4305
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Protein Quantitation: Bradford Spot Test
Ed Harlow and David Lane
CSH Protocols; 2006; doi:10.1101/pdb.prot4306
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Protein Quantitation: UV Detection
Ed Harlow and David Lane
CSH Protocols; 2006; doi:10.1101/pdb.prot4307
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Bulk Precipitation of Proteins by Ammonium Sulfate
Richard J. Simpson
CSH Protocols; 2006; doi:10.1101/pdb.prot4308
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Fractional Precipitation of Proteins by Ammonium Sulfate
Richard J. Simpson
CSH Protocols; 2006; doi:10.1101/pdb.prot4309
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Precipitation of Proteins by Organic Solvents
Richard J. Simpson
CSH Protocols; 2006; doi:10.1101/pdb.prot4310
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Precipitation of Proteins by Polyethylene Glycol
Richard J. Simpson
CSH Protocols; 2006; doi:10.1101/pdb.prot4311
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Precipitation of Proteins by Polyethylenimine
Richard J. Simpson
CSH Protocols; 2006; doi:10.1101/pdb.prot4312
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SDS-PAGE of Proteins
Richard J. Simpson
CSH Protocols; 2006; doi:10.1101/pdb.prot4313
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Electrophoresis of Peptides (Tricine-SDS-PAGE)
Richard J. Simpson
CSH Protocols; 2006; doi:10.1101/pdb.prot4314
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Ovariectomy for Induction of Blastocyst Implantation Delay
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols; 2006; doi:10.1101/pdb.prot4378
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Simple, Reliable Steps for DNA Fragment Isolation and Purification
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols; 2006; doi:10.1101/pdb.prot4389
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Isolating Bacterial Artificial Chromosome (BAC) DNA from Bacterial Cultures Using the NucleoBond System
Thom Saunders
CSH Protocols; 2006; doi:10.1101/pdb.prot4390
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Purification of Bacterial Artificial Chromosome (BAC) DNA Prepared with the NucleoBond System
Thom Saunders
CSH Protocols; 2006; doi:10.1101/pdb.prot4391
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Large-Scale Preparation of Agarose Plugs of Yeast DNA
Lluis Montoliu
CSH Protocols; 2006; doi:10.1101/pdb.prot4392
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Purification of Yeast Artificial Chromosome (YAC) DNA with Filtration Units
Lluis Montoliu
CSH Protocols; 2006; doi:10.1101/pdb.prot4393
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Making Holding Pipettes
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols; 2006; doi:10.1101/pdb.prot4394
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Making Injection Pipettes
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols; 2006; doi:10.1101/pdb.prot4395
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Microinjection Setup
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols; 2006; doi:10.1101/pdb.prot4396
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Microinjection of Mouse Zygotes
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols; 2006; doi:10.1101/pdb.prot4397
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Preparing Mouse Embryo Fibroblasts
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols; 2006; doi:10.1101/pdb.prot4398
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Preparing Feeder Cell Layers from STO or Mouse Embryo Fibroblast (MEF) Cells: Treatment with Mitomycin C
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols; 2006; doi:10.1101/pdb.prot4399
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Preparing Feeder Cell Layers from STO or Mouse Embryo Fibroblast (MEF) Cells: Treatment with 
-Irradiation
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols; 2006; doi:10.1101/pdb.prot4400
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Passage of Embryonic Stem (ES) Cells
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols; 2006; doi:10.1101/pdb.prot4401
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Freezing and Thawing of Embryonic Stem (ES) Cells Using Cryovials
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols; 2006; doi:10.1101/pdb.prot4402
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De Novo Isolation of Embryonic Stem (ES) Cell Lines from Blastocysts
Andras Nagy, Marina Gertsenstein, Kristina Vintersten, and Richard Behringer
CSH Protocols; 2006; doi:10.1101/pdb.prot4403
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